4/17/2024 0 Comments Spectrum tv channel list 16946Although there is no gold standard for identifying anti-SARS-CoV-2 seropositivity, aggregate standards can be used to assess seropositivity. IMPORTANCE We describe the qualitative test characteristics of the Abbott SARS-CoV-2 IgG II Quant assay against four other anti-SARS-CoV-2 IgG assays commonly used in Canada. Our next steps are to assess how well the Abbott anti-S assays quantitatively detect wild-type and SARS-CoV-2 variants of concern. Our study provides context on the use of commonly used SARS-CoV-2 serologies in Canada and identifies how these assays qualitatively compare to newer commercial assays. The specificity of the Abbott anti-S assay was 99.35% (95% CI, 99.21 to 99.46%). Using this composite reference standard approach, the sensitivity of the Abbott anti-S assay was 95.96% (95% confidence interval, 93.27 to 97.63%). We then scored each previously characterized specimen as positive when two anti-SARS-COV-2 assays identified anti-SARS-CoV-2 IgG in the specimen. The strongest qualitative relationship was between Sinai RBD and the Abbott anti-S assay (kappa, 0.707 standard error of kappa, 0.018 95% confidence interval, 0.671 to 0.743). The four comparator assays were the Abbott anti-nucleocapsid (N) assay and three commonly used Canadian in-house IgG enzyme-linked immunosorbent assays (ELISAs) recognizing distinct recombinant viral antigens, full-length spike glycoprotein, glycoprotein RBD, and nucleocapsid. The Abbott anti-S assay has a qualitative threshold of 50 AU/mL. The analysis compared the characteristics of the Abbott SARS-CoV-2 IgG II Quant assay (Abbott anti-spike, Abbott, Chicago, IL) against four other IgG assays. In this current study, we analyzed 17,428 available and previously characterized retention samples collected from April 2020 to March 2021. Our group has previously used laboratory and commercially developed assays to understand the IgG responses to SARS-CoV-2 antigens, including nucleocapsid (N), spike (S), and receptor binding domain (RBD), in Canadian blood donors.
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